Thermo Fisher Scientific STAT5a Polyclonal Antibody
STAT5a 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody입니다. Western blot, IHC, IP 등 다양한 응용에 적합하며, 비결합형 액상 형태로 제공됩니다. 4°C 보관, 1년 권장 보관 기간.
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Thermo Fisher Scientific STAT5a Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 744 and 794 of Human Signal Transducer and Activator of Transcription 5A |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
STAT5 alpha is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by receptor-associated kinases, then form homo- or heterodimers that translocate to the nucleus to act as transcription activators.
STAT5 alpha mediates responses to ligands such as IL2, IL3, IL7, GM-CSF, erythropoietin, thrombopoietin, and various growth hormones. Activation of STAT5 alpha in myeloma and lymphoma associated with TEL/JAK2 gene fusion is independent of cell stimulus and is essential for tumorigenesis.
In mouse, STAT5 alpha induces expression of BCL2L1/BCL-X(L), suggesting anti-apoptotic function. STAT5 alpha and STAT5 beta share 96% sequence similarity and are co-expressed in most tissues, though differential accumulation is observed in muscle and mammary tissue. STAT5 alpha plays critical roles in reproduction, lactation, immune function, and somatic growth.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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