Thermo Fisher Scientific Arginase 1 Monoclonal Antibody (A1exF5), PE-Cyanine7, eBioscience

Thermo Fisher Scientific Arginase 1 Monoclonal Antibody (A1exF5), PE-Cyanine7, eBioscience™

Applications and Tested Dilution

Product Specifications

Available Formats

• Alexa Fluor 488
• Alexa Fluor 700
• APC
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 650
• Brilliant Violet 711
• eFluor 450
• PE
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The monoclonal antibody A1exF5 recognizes both human and mouse Arginase 1, a cytosolic enzyme (Arg1).
This clone is compatible with standard intracellular staining protocols and the Foxp3/Transcription Factor Staining Buffer Set.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometry using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00).
Refer to BestProtocols®: Protocol A for cytoplasmic intracellular proteins.

Use ≤0.5 µg per test (100 µL final volume). Cell number may vary (10⁵–10⁸ cells/test). Antibody titration recommended for optimal performance.

Light Sensitivity: Protect vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer ([Product # 00-8222]) or 1-step Fix/Lyse Solution ([Product # 00-5333-54]) for up to 3 days at 4°C in the dark with minimal effect on brightness and FRET efficiency.

Excitation: 488–561 nm
Emission: 775 nm
Compatible Lasers: Blue, Green, Yellow-Green

Target Information

Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, the final step in the urea cycle.
It regulates nitric oxide production by depriving NO synthase of its substrate.
Arginase 1 is expressed in liver, neutrophils, myeloid-derived suppressor cells (MDSC), and neural stem cells.
In mice, it is a hallmark of alternatively activated macrophages (M2a).
Defects in Arginase 1 cause argininemia, an autosomal recessive disorder characterized by hyperammonemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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