Thermo Fisher Scientific Arginase 1 Monoclonal Antibody (A1exF5), PE-Cyanine7, eBioscience
인간 및 마우스 Arginase 1을 인식하는 단클론 항체로, PE-Cyanine7 형광체가 결합된 형광 Flow Cytometry용 시약. 0.5 µg/test로 사용 가능하며, 세포 내 염색 및 Foxp3/Transcription Factor Staining Buffer 세트와 호환. 빛에 민감하여 암실 보관 필요.
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Thermo Fisher Scientific Arginase 1 Monoclonal Antibody (A1exF5), PE-Cyanine7, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | View 27 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Fruit fly, Mouse, Rat |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine7, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | A1exF5 |
| Immunogen | E.coli-derived recombinant mouse Arginase 1 |
| Conjugate | PE-Cyanine7 |
| Excitation/Emission Max | 569 / 780 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2734840 |
Available Formats
- Alexa Fluor 488
- Alexa Fluor 700
- APC
- Brilliant UV 805
- Brilliant Violet 421
- Brilliant Violet 480
- Brilliant Violet 650
- Brilliant Violet 711
- eFluor 450
- PE
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
The monoclonal antibody A1exF5 recognizes both human and mouse Arginase 1, a cytosolic enzyme (Arg1).
This clone is compatible with standard intracellular staining protocols and the Foxp3/Transcription Factor Staining Buffer Set.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometry using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00).
Refer to BestProtocols®: Protocol A for cytoplasmic intracellular proteins.
Use ≤0.5 µg per test (100 µL final volume). Cell number may vary (10⁵–10⁸ cells/test). Antibody titration recommended for optimal performance.
Light Sensitivity: Protect vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer ([Product # 00-8222]) or 1-step Fix/Lyse Solution ([Product # 00-5333-54]) for up to 3 days at 4°C in the dark with minimal effect on brightness and FRET efficiency.
Excitation: 488–561 nm
Emission: 775 nm
Compatible Lasers: Blue, Green, Yellow-Green
Target Information
Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, the final step in the urea cycle.
It regulates nitric oxide production by depriving NO synthase of its substrate.
Arginase 1 is expressed in liver, neutrophils, myeloid-derived suppressor cells (MDSC), and neural stem cells.
In mice, it is a hallmark of alternatively activated macrophages (M2a).
Defects in Arginase 1 cause argininemia, an autosomal recessive disorder characterized by hyperammonemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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