
Thermo Fisher Scientific GTF3C3/TFIIIC102 Polyclonal Antibody
Rabbit polyclonal antibody targeting human GTF3C3 (TFIIIC102), validated for WB, IHC, and IP applications. High specificity via antigen affinity purification. Recommended for research use only, with one-year shelf life and storage at 4°C.
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Thermo Fisher Scientific GTF3C3/TFIIIC102 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:200–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 475 and 525 of human general transcription factor 3C polypeptide 3 (TFIIIC 102 kDa subunit) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
RNA polymerase III synthesizes tRNA, 5S rRNA, 7SL RNA, and U6 snRNA, and is overexpressed in many transformed cell lines and tumors. This high expression supports rapid cell growth and division. The TFIIIC2 complex, composed of TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90, and TFIIIC63, recruits RNA polymerase III and TFIIIB to promoter elements and plays a key role in malignant cell deregulation. TFIIIB includes TBP, BRF1, and TFIIIB itself, and its expression is upregulated in transformed cells. Tumor suppressors such as RB and p53 are often inactivated in carcinomas, reducing their ability to bind and inhibit TFIIIB function.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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