Thermo Fisher Scientific BPI Polyclonal Antibody

Applications

• Immunohistochemistry (Paraffin) (IHC (P)): 1:500–1:1,000

Product Specifications

Product Specific Information

• Immunogen sequence:
  TTGSMEVSAE SNRLVGELKL DRLLLELKHS NIGPFPVELL QDIMNYIVPI LVLPRVNEKL QKGFPLPTPA RVQLYNVVLQ P
• Highest antigen sequence identity to orthologs: Mouse - 57%, Rat - 59%

Target Information

The antimicrobial protein BPI is a 55 kDa protein found in the primary (azurophilic) granules of human neutrophils and also detected on the surface of neutrophils, small intestinal, and oral epithelial cells.
BPI is a bactericidal compound present in polymorphonuclear cells (PMN) and at lower levels in eosinophils. It exhibits high affinity for the lipid A region of lipopolysaccharides (LPS) in gram-negative bacteria, leading to multiple anti-infective activities including:

• Cytotoxicity via sequential damage to bacterial membranes
• Neutralization of gram-negative bacterial LPS
• Opsonization to enhance phagocytosis by neutrophils

BPI may play a critical role in LPS-triggered airway disease, as airway epithelial cells express the BPI gene and produce the protein. Inflammation induced by LPS may contribute to rapid airflow decline, a severe complication of hematopoietic cell transplantation.
Additionally, a 21 kDa recombinant fragment of BPI (rBPI21) confers survival advantage against invasive pneumococcal disease by binding to pneumolysin from gram-positive bacteria.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.