Thermo Fisher Scientific PTK2 Polyclonal Antibody, MaxPab

Applications

Western Blot (WB)

• Tested Dilution: 1–5 µg/mL
• Publications: -

in situ PLA (PLA)

• Tested Dilution: 1:1200
• Publications: -

Product Specifications

Product Specific Information

Sequence of this protein is as follows:
MKYQEVRCLT SFNISVSFPA ETDDYAEIID EEDTYTMPST RDYEIQRERI ELGRCIGEGQ FGDVHQGIYM SPENPALAVA IKTCKNCTSD SVREKFLQEA LTMRQFDHPH IVKLIGVITE NPVWIIMELC TLGELRSFLQ VRKYSLDLAS LILYAYQLST ALAYLESKRF VHRDIAARNV LVSSNDCVKL GDFGLSRYME DSTYYKASKG KLPIKWMAPE SINFRRFTSA SDVWMFGVCM WEILMHGVKP FQGVKNNDVI GRIENGERLP MPPNCPPTLY SLMTKCWAYD PSRRPRFTEL KAQLSTILEE EKAQQEERMR MESRRQATVS WDSGGSDEAP PKPSRPGYPS PRSSEGFYPS PQHMVQTNHY QVSGYPGSHG ITAMAGSIYP GQASLLDQTD SWNHRPQEIA MWQPNVEDST VLDLRGIGQV LPTHLMEERL IRQQQEMEED QRWLEKEERF LKPDVRLSRG SIDREDGSLQ GPIGNQHIYQ PVGKPDPAAP PKKPPRPGAP GHLGSLASLS SPADSYNEGV KLKPQEISPP PTANLDRSND KVYENVTGLV KAVIEMSSKI QPAPPEEYVP MVKEVGLALR TLLATVDETI PLLPASTHRE IEMAQKLLNS DLGELINKMK LAQQYVMTSL QQEYKKQMLT AAHALAVDAK NLLDVIDQAR LKMLGQTRRH

Target Information

Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle.

FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin.

Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, resulting in activation of ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin.

In vitro, the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site-directed mutagenesis. Several transcript variants encoding different isoforms have been found for the FAK gene, but the full-length natures of only three of them have been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.