Thermo Fisher Scientific PTK2 Polyclonal Antibody, MaxPab

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

-

in situ PLA (PLA)

1:1200

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PTK2 (AAH28733.1, 1 a.a. approximately 680 a.a) full-length human protein. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:FAK,uniProtId:Q05397-1,ncbiNodeId:9606,antigenRange:1-1052,antigenLength:1052,antigenImageFileName:H00005747-D01P_FAK_Q05397-1_Rabbit.svg,antigenImageFileNamePDP:H00005747-D01P_FAK_Q05397-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Sequence of this protein is as follows: MKYQEVRCLT SFNISVSFPA ETDDYAEIID EEDTYTMPST RDYEIQRERI ELGRCIGEGQ FGDVHQGIYM SPENPALAVA IKTCKNCTSD SVREKFLQEA LTMRQFDHPH IVKLIGVITE NPVWIIMELC TLGELRSFLQ VRKYSLDLAS LILYAYQLST ALAYLESKRF VHRDIAARNV LVSSNDCVKL GDFGLSRYME DSTYYKASKG KLPIKWMAPE SINFRRFTSA SDVWMFGVCM WEILMHGVKP FQGVKNNDVI GRIENGERLP MPPNCPPTLY SLMTKCWAYD PSRRPRFTEL KAQLSTILEE EKAQQEERMR MESRRQATVS WDSGGSDEAP PKPSRPGYPS PRSSEGFYPS PQHMVQTNHY QVSGYPGSHG ITAMAGSIYP GQASLLDQTD SWNHRPQEIA MWQPNVEDST VLDLRGIGQV LPTHLMEERL IRQQQEMEED QRWLEKEERF LKPDVRLSRG SIDREDGSLQ GPIGNQHIYQ PVGKPDPAAP PKKPPRPGAP GHLGSLASLS SPADSYNEGV KLKPQEISPP PTANLDRSND KVYENVTGLV KAVIEMSSKI QPAPPEEYVP MVKEVGLALR TLLATVDETI PLLPASTHRE IEMAQKLLNS DLGELINKMK LAQQYVMTSL QQEYKKQMLT AAHALAVDAK NLLDVIDQAR LKMLGQTRRH

Target Information

Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis. Several transcript variants encoding different isoforms have been found for the FAK gene, but the full-length natures of only three of them have been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.