Thermo Fisher Scientific PTK2 Monoclonal Antibody (1C1)

Applications

Western Blot (WB)

• Tested Dilution: 1–5 µg/mL

ELISA

• Tested Dilution: 0.1 ng/mL

Product Specifications

Product Specific Information

Sequence of this protein:
EGFYPSPQHM VQTNHYQVSG YPGSHGITAM AGSIYPGQAS LLDQTDSWNH RPQEIAMWQP NVEDSTVLDL RGIGQVLPTH LMEERLIRQQ QEMEEDQRWL EKEERFLKPD VRLSRGSIDR EDGSLQGPIG NQHIYQ

Target Information

Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling.
FAK plays an important role in:

• Cell spreading, differentiation, migration, and cell death
• Acceleration of the G1 to S phase transition of the cell cycle

FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, where cells attach to the extracellular matrix via integrin receptors.
Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK, resulting in ERK2 MAP kinase activation.
In v-Src-transformed NIH3T3 cells, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin.
In vitro, the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK at Tyr925, identified by site-directed mutagenesis.
Several transcript variants encoding different isoforms have been found for the FAK gene, with three full-length forms determined.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.