Thermo Fisher Scientific Calcineurin B Polyclonal Antibody
Calcineurin B 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, ICC/IF, ELISA에 적합합니다. Human, Mouse, Rat 시료에 반응하며, 고순도 Affinity Chromatography 정제 항체입니다. PBS/glycerol buffer에 보관되며 -20°C에서 안정적입니다.
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Calcineurin B Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 11–170 of human PPP3R1 (NP_000936.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.93 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2804229 |
Product Specific Information
Immunogen sequence:
MCSHFDADEI KRLGKRFKKL DLDNSGSLSV EEFMSLPELQ QNPLVQRVID IFDTDGNGEV DFKEFIEGVS QFSVKGDKEQ KLRFAFRIYD MDKDGYISNG ELFQVLKMMV GNNLKDTQLQ QIVDKTIINA DKDGDGRISF EEFCAVVGGL DIHKKMVVDV
Positive Samples:
Mouse brain, Mouse spleen, Mouse lung, Rat brain, SH-SY5Y, SW480, HL-60, MCF7, Rat testis
Cellular Location:
Cell membrane, Cytoplasm, Lipid-anchor, Membrane, Cytosol, Sarcolemma
Target Information
Calcineurin is a Ser/Thr, calcium and calmodulin-dependent protein phosphatase that plays an essential role in the T-cell activation pathway. It consists of two subunits:
- Calcineurin A (CnA): 60 kDa catalytic subunit, homologous to protein phosphatases 1 and 2A
- Calcineurin B (CnB): 19 kDa regulatory subunit containing four EF-hand calcium-binding sites
Calcineurin-mediated dephosphorylation of NF-AT is crucial for its activation, nuclear translocation, and gene expression in T-cells. Immunosuppressive drugs such as cyclosporin A and FK506 inhibit NF-AT activation by forming complexes with immunophilins that block substrate access to CnA’s catalytic domain.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.