Thermo Fisher Scientific Calcineurin B Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Immunogen sequence:
MCSHFDADEI KRLGKRFKKL DLDNSGSLSV EEFMSLPELQ QNPLVQRVID IFDTDGNGEV DFKEFIEGVS QFSVKGDKEQ KLRFAFRIYD MDKDGYISNG ELFQVLKMMV GNNLKDTQLQ QIVDKTIINA DKDGDGRISF EEFCAVVGGL DIHKKMVVDV

Positive Samples:
Mouse brain, Mouse spleen, Mouse lung, Rat brain, SH-SY5Y, SW480, HL-60, MCF7, Rat testis

Cellular Location:
Cell membrane, Cytoplasm, Lipid-anchor, Membrane, Cytosol, Sarcolemma

Target Information

Calcineurin is a Ser/Thr, calcium and calmodulin-dependent protein phosphatase that plays an essential role in the T-cell activation pathway. It consists of two subunits:

• Calcineurin A (CnA): 60 kDa catalytic subunit, homologous to protein phosphatases 1 and 2A
• Calcineurin B (CnB): 19 kDa regulatory subunit containing four EF-hand calcium-binding sites

Calcineurin-mediated dephosphorylation of NF-AT is crucial for its activation, nuclear translocation, and gene expression in T-cells. Immunosuppressive drugs such as cyclosporin A and FK506 inhibit NF-AT activation by forming complexes with immunophilins that block substrate access to CnA’s catalytic domain.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.