Thermo Fisher Scientific IFN omega Human ProcartaPlex Simplex Kit

Invitrogen™ IFN omega Human ProcartaPlex™ Simplex Kit

The Human IFN omega Simplex ProcartaPlex Kit measures IFN omega protein and is designed to be combinable with other Simplex kits so you can create your own multiplex panel utilizing Luminex xMAP technology for protein detection and quantitation. When combining multiple Simplex kits (i.e., when not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed per assay plate regardless of plex size.

About ProcartaPlex Assays for the Luminex Platform

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies that bind to different epitopes of one protein to quantitate multiple protein targets simultaneously using a Luminex instrument.
These multiplex assays require as little as 25 μL of plasma or serum, or 50 μL of cell culture supernatant, and deliver analyzed results in about four hours.

Key Features

• More results per sample: Measure multiple protein targets simultaneously in a single 25–50 μL sample
• Well-established Luminex technology: Highly referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 μL sample from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create spectrally unique signatures identifiable by Luminex xMAP detection systems (e.g., Luminex 200, FLEXMAP 3D, and MAGPIX).
Similar to a sandwich ELISA, matched antibody pairs identify the protein of interest. In a multiplexed assay, each bead is labeled with antibodies specific for a single target protein, and bound proteins are detected with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE).

The key difference between a ProcartaPlex assay and a traditional ELISA is that the capture antibody in ProcartaPlex is conjugated to a bead rather than adsorbed to a microplate well, allowing reagents to remain free-floating in solution. Detection is performed by instruments such as the Luminex 200, which uses two lasers—one to distinguish bead spectral signatures and another to quantify RPE fluorescence proportional to protein concentration.

ProcartaPlex multiplex assays enable profiling of more target proteins using less sample and time compared to conventional sandwich ELISA.
Multiplex panels are available across six species (human, mouse, rat, nonhuman primate, porcine, and canine).
Visit thermofisher.com/procartaplex for more information and a comprehensive list of available protein targets.

제품 사양 (Specifications)