Thermo Fisher Scientific MCT8 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000–1:10,000

Immunoprecipitation (IP)

• Tested Dilution: 2–10 µg/mg lysate

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt.

Target Information

Monocarboxylates such as lactate and pyruvate play an integral role in cellular metabolism. Lactic acid is produced in large quantities as a result of glycolysis, which provides the majority of ATP to cells under normal physiological conditions. However, accumulation of lactic acid leads to a decrease in intracellular pH and cessation of glycolysis. To maintain glycolytic activity, lactic acid must be transported out of the cell via monocarboxylate transporters (MCTs), which act as proton symports and are stereoselective for L-lactate.
The MCT family consists of at least eight members (MCT1–8), each containing 10–12 transmembrane-helical domains, with both termini in the cytoplasm.
Defects in the gene encoding MCT8 (SLC16A2) can cause monocarboxylate transporter 8 deficiency, an X-linked disorder affecting hormone transport and leading to severe psychomotor retardation and abnormal thyroid levels.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.