Thermo Fisher Scientific Chorionic Gonadotropin Monoclonal Antibody (FB12), Alexa Fluor 488, eBioscience

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

-

Immunocytochemistry (ICC/IF)

20 µg/mL

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Product Specifications

Species Reactivity

Human

Published species

Not Applicable

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

FB12

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

View additional formats

• Unconjugated
• Request custom conjugation

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10854569

Product Specific Information

Description: This FB12 monoclonal antibody reacts with human Chorionic Gonadotropin (hCG). This secreted glycoprotein is composed of an alpha subunit, which is common to other peptide hormones such as luteinizing hormone (LH), follicle stimulating hormone (FSH) and thyroid stimulating hormone (TSH), and a beta subunit which determines the specificity of the hormone. FB12 recognizes both the intact hCG (composed of the alpha and beta subunits) as well as the free beta subunit, highly expressed in both choriocarcinoma and the serum of pregnant women. hCG is normally expressed by trophoblastoid cells (syncytiotrophoblasts and extravillous cytotrophoblasts) that are responsible for maintenance of the corpus luteum during early gestation. hCG plays an important role in the regulation of angiogenesis through activation of the PI3K/mTOR pathway, resulting in upregulation of vascular endothelial growth factor (VEGF). Ectoptic expression of hCG has also been reported in multiple types of cancer (bladder, lung, breast, pancreas, renal).

Applications Reported: This FB12 antibody has been reported for use in immunohistochemical staining of frozen tissue sections (IHC-F) and immunocytochemistry (ICC).

Applications Tested: This FB12 antibody has been tested by immunocytochemistry on fixed BeWo cells. This can be used at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Human chorionic gonadotropin (hCG) is a placental hormone that stimulates secretion of the pregnancy-sustaining steroid progesterone. hCG is a member of a family of glycoprotein hormones including pituitary hormones lutropin (luteinizing hormone;LH), follitropin (follicle-stimulating hormone; FSH) and thyrotropin (thyroid-stimulating hormone; TSH). Members of this family are all composed of disulfide-rich heterodimers with two dissimilar subunits namely alpha- and beta-subunits. The alpha subunit is common to all the members of the family within a given species. The beta subunits are distinct in each of the horomones and confer the receptor and biological specificity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.