Thermo Fisher Scientific CD8a Monoclonal Antibody (RPA-T8), NovaFluor Blue 610-70S, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.2 µg)/test

Product Specifications

Additional Available Formats

• Alexa Fluor 532, 561, 660, 700
• APC, APC-eFluor 780
• Brilliant UV 395, 563, 615, 661, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 450, 506
• FITC, PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
• PE-eFluor 610, PerCP-Cyanine5.5
• Super Bright 436, 600, 645, 702, 780
• NovaFluor Blue 660-120S, Red 710, Yellow 610, Yellow 660
• Custom conjugation available upon request

Product Specific Information

The RPA-T8 monoclonal antibody reacts with the human CD8a molecule, a 32–34 kDa cell surface receptor expressed as a CD8α/β heterodimer or CD8α/α homodimer. CD8a is found on most thymocytes and subsets of mature T and NK cells. It binds to MHC class I and associates with protein tyrosine kinase p56lck, playing a key role in T-cell development and activation.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and validated for human peripheral blood cells at 5 µL (0.02 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains with NovaFluor dyes.
• Use CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) for optimal staining.
  • Add 5 µL per sample before mixing with cells.
  • For single-color controls, use 5 µL per 100 µL cell sample (10³–10⁸ cells).

Technology:
NovaFluor conjugates utilize Phiton™ technology with engineered nucleic acid dye structures to minimize spillover and spread effects.
Excitation: 509 nm
Emission: 614 nm
Laser: 488 nm (Blue)

Target Information

CD8 (Cluster of Differentiation 8) is a type I transmembrane glycoprotein of the immunoglobulin receptor family, essential in T-cell signal transduction, differentiation, and activation. It is expressed predominantly on T cells as a CD8α/β heterodimer, functioning as a co-receptor with the TCR for MHC class I molecules. CD8α is also found as a homodimer on subsets of T cells, NK cells, monocytes, and dendritic cells. Engagement with MHC-I/peptide complexes triggers Lck kinase recruitment, leading to cytokine production, motility, and activation of cytotoxic T lymphocytes (CTLs) for pathogen and tumor cell clearance. Differentiation of naïve CD8⁺ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for diagnostic use or resale without authorization.

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