Himedia Triple Sugar Iron Agar

Himedia Triple Sugar Iron Agar

Originally proposed by Sulkin and Willett and modified by Hajna for identifying Enterobacteriaceae.
This medium complies with the recommendation of APHA for the examination of:

• Meat and food products
• Milk and dairy products
• Microbial limit test for confirming the presence of Salmonellae
• Identification of gram-negative bacilli

Reaction Interpretation

• Alkaline slant / acid butt: Only glucose fermented
• Acid slant / acid butt: Dextrose and sucrose fermented, or dextrose and lactose fermented, or all three sugars (dextrose, lactose, sucrose) fermented
• Bubbles or cracks present: Gas production
• Black precipitate present: H₂S gas production

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