Thermo Fisher Scientific Involucrin Polyclonal Antibody
Western blot, IHC, ICC 등 다양한 응용에 적합한 Involucrin 폴리클로날 항체. 사람 및 생쥐 반응성, Rabbit IgG 유래. 고순도 친화 크로마토그래피 정제, PBS/glycerol 완충액 보관. 각질세포 분화 연구에 유용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (IHC) | 1:50–1:200 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human IVL (Accession P07476), corresponding to amino acid residues A548–K585 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2853754 |
Product Specific Information
This antibody detects endogenous levels of total Involucrin.
Target Information
Involucrin is a component of the keratinocyte crosslinked envelope, found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. It is expressed in a range of stratified squamous epithelia, including the cornea.
In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, it is expressed by all cells that have left the basal layer.
Involucrin expression is abnormal in squamous cell carcinomas and premalignant lesions and is reduced in severe dysplasias of the larynx and cervix. Its gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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