Thermo Fisher Scientific Phospho-AMPK alpha-1 (Ser485) Monoclonal Antibody (E.429.3)
Phospho-AMPK alpha-1 (Ser485) 단일클론 항체로, 인간 및 설치류 시료에 적합. Western blot에 최적화되어 있으며, AMPKα2 등 관련 단백질과 교차 반응 없음. 고순도 친화 크로마토그래피 정제 및 안정한 액상 포맷 제공.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | E.429.3 |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding pSer485 of human AMPKα1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 117 µg/mL |
| Purification | Affinity chromatography |
| Storage buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | <0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_10980450 |
Product Specific Information
- It is not recommended to aliquot this antibody.
- This antibody is not cross-reactive with phosphorylated AMPK-alpha2 or other related proteins.
Target Information
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the γ phosphate of ATP, onto an acceptor amino acid in a substrate protein.
By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating:
- Cellular metabolism
- Transcription
- Cell cycle progression
- Cytoskeletal rearrangement and cell movement
- Apoptosis and differentiation
With more than 500 gene products, the protein kinase family is one of the largest in eukaryotes. The family has been classified into 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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