Thermo Fisher Scientific MCM10 Polyclonal Antibody
Rabbit polyclonal antibody targeting human MCM10 protein for ICC/IF applications. Recombinant MCM10 immunogen-based, antigen affinity purified, and supplied as unconjugated liquid form. Ideal for studying DNA replication initiation and MCM complex inte...
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Applications
Immunocytochemistry (ICC/IF)
- Tested Dilution: 0.25–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Human MCM10 (Recombinant protein control fragment, Product #RP-105551) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.05 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C (avoid freeze/thaw cycles) |
| Shipping Conditions | Wet ice |
| RRID | AB_2663818 |
Product Specific Information
Immunogen sequence:
REKLEEIDWVT FGVILKKVTP QSVNSGKTFS IWKLNDLRDL TQCVSLFLFG EVHKALWKTE QGTVVGILNA NPMK
- Highest antigen sequence identity to orthologs: mouse 84%, rat 88%
Target Information
The MCM10 protein is one of the highly conserved mini-chromosome maintenance proteins involved in the initiation of eukaryotic genome replication. The hexameric MCM complex is a key component of the pre-replication complex (pre-RC), contributing to replication fork formation and recruitment of other DNA replication-related proteins.
MCM10 interacts with MCM2, MCM6, and ORC2, and is regulated by proteolysis and phosphorylation in a cell cycle-dependent manner. Studies in Xenopus indicate that chromatin binding occurs after pre-RC assembly and before origin unwinding.
Alternatively spliced transcript variants encoding distinct isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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