Thermo Fisher Scientific PKLR Polyclonal Antibody
Thermo Fisher Scientific의 PKLR Polyclonal Antibody는 인간 및 랫트 반응성을 가지며 Western blot과 IHC(P)에서 사용 가능. 항원 친화 크로마토그래피로 정제된 액상 항체로, 안정적인 농도(1.01 mg/mL)와 높은 특이성을 제공. 연구용으로만 사용.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:3,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:100–1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 178–550 of Human Pyruvate Kinase |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.01 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | 0.1M Tris glycine, pH 7, with 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2544781 |
Product Specific Information
- Recommended positive controls: Jurkat, Raji, K562, rat liver, PKLR-transfected 293T
- Predicted reactivity: Mouse (93%), Rat (92%), Xenopus laevis (80%), Dog (93%), Bovine (94%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phosphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis.
Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA).
Multiple transcript variants encoding different isoforms have been found for this gene.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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