Thermo Fisher Scientific CD4 Monoclonal Antibody (OKT4 (OKT-4)), eFluor 660, eBioscience
인간 CD4 항원에 특이적인 OKT4 단클론 항체로, eFluor 660 형광체가 결합된 제품입니다. Flow Cytometry에 최적화되어 있으며, HIV 수용체 연구 및 T-helper 세포 분석에 사용됩니다. 4°C에서 암소 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.03 µg)/test | View 24 publications |
Product Specifications
| Item | Specification |
|---|---|
| Species Reactivity | Human |
| Published Species | Hamster, Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), eFluor™ 660, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OKT4 (OKT-4) |
| Conjugate | eFluor™ 660 |
| Excitation / Emission Max | 651 / 668 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574153 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 661
- Brilliant Violet 650
- Brilliant Violet 786
- eFluor 450
- FITC
- Functional Grade
- NovaFluor Blue 610-70S
- NovaFluor Blue 660-120S
- NovaFluor Blue 690
- NovaFluor Yellow 660
- PE
- PE-Cyanine7
- PerCP-Cyanine5.5
- Super Bright 436
- Super Bright 600
- Super Bright 702
- Request custom conjugation
Product Specific Information
The OKT4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface glycoprotein expressed by thymocytes, T-helper cells, and at low levels on monocytes. CD4 serves as a receptor for HIV. The OKT4 antibody recognizes a different epitope than RPA-T4 and does not cross-block binding.
Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and validated for flow cytometry using human peripheral blood cells. Recommended usage: 5 µL (0.03 µg) per test in 100 µL final volume. Cell count range: 10⁵–10⁸ cells/test.
Fluorophore Information:
eFluor® 660 replaces Alexa Fluor® 647. Excitation: 633–647 nm; Emission: 668 nm; Laser: Red.
Ensure instrument compatibility with this fluorochrome.
Post-manufacturing filtration: 0.2 µm.
Target Information
CD4 antigen is involved in MHC class II molecule recognition and acts as a co-receptor for HIV. Primarily expressed on T-helper lymphocytes, CD4 is also found on monocytes, macrophages, and dendritic cells. Tissue expression includes thymus, lymph nodes, tonsils, spleen, and certain non-lymphoid tissues such as brain and gut. CD4 contributes to early T-cell activation via association with the T-cell receptor complex and Lck kinase. It may also mediate neuronal damage in immune-related CNS diseases. Multiple alternatively spliced transcripts exist (RefSeq, July 2017).
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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