Thermo Fisher Scientific CDKN2A Monoclonal Antibody (OTI8A1), TrueMAB

Applications

• Western Blot (WB): 1:200–1:1,000

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

The CDKN2A gene produces several transcript variants with distinct first exons. At least three alternatively spliced variants encode different proteins, two of which are CDK4 kinase inhibitors. Another transcript, located 20 Kb upstream, encodes an alternate open reading frame (ARF) product that stabilizes the tumor suppressor p53 by binding and sequestering MDM2, an E3 ubiquitin-protein ligase responsible for p53 degradation.
Despite structural differences, both CDK inhibitor isoforms and the ARF product regulate cell cycle G1 progression through CDK4 and p53 pathways.
Mutations or deletions of this gene are common in various tumors, highlighting its role as a critical tumor suppressor.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.