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Tet-One systems provide all necessary components for tetracycline-inducible (tet) expression on a single vector delivered by AAV2 virus. The Tet-On 3G transactivator is expressed from the human phosphoglycerate kinase 1 (PGK) promoter, and the cloned gene of interest is expressed from the PTRE3GS promoter. The AAVpro system contains the reagents to generate the Tet-One vector, as well as components to efficiently package and isolate AAV2 particles.
Tet-One systems provide all necessary components for tetracycline-inducible (tet) expression on a single vector delivered by AAV2 virus. The Tet-On 3G transactivator is expressed from the human phosphoglycerate kinase 1 (PGK) promoter, and the cloned gene of interest is expressed from the PTRE3GS promoter. The AAVpro system contains the reagents to generate the Tet-One vector, as well as components to efficiently package and isolate AAV2 particles. This kit uses helper-free packaging, a unique system for preparing high-titer AAV2 particles without the use of a helper virus.
There are also several other Tet-One vector options for mammalian cells.
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