Thermo Fisher Scientific CD117 (c-Kit) Monoclonal Antibody (2B8), PE-Cyanine7, eBioscience
CD117 (c-Kit) 단일클론 항체(2B8), PE-Cyanine7 결합형으로 마우스 CD117 검출에 적합. 유세포 분석에 최적화되어 있으며, 광민감성 주의 필요. 4°C 암소 보관, 헤마토포이에틱 전구세포 및 비만세포 연구용.
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Thermo Fisher Scientific CD117 (c-Kit) Monoclonal Antibody (2B8), PE-Cyanine7, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 1 µg/test | View 107 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Pig |
| Published Species | Fish, Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), PE-Cyanine7, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2B8 |
| Conjugate | PE-Cyanine7 |
| Excitation / Emission Max | 569 / 780 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_469643 |
Additional Formats Available
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 563 / 615 / 661 / 737
- Brilliant Violet 421 / 480 / 605 / 650 / 786
- eFluor 450
- FITC
- PE / PE-Cyanine5 / PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436 / 600 / 645 / 702 / 780
- Custom conjugation available upon request
Product Specific Information
The 2B8 monoclonal antibody reacts with mouse CD117 (c-Kit receptor, Steel factor receptor, stem cell factor receptor), a member of the tyrosine kinase receptor family. This 145-kDa molecule is expressed by most hematopoietic progenitor cells in mouse bone marrow and also by mast cells.
Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of mouse bone marrow cells; optimal use ≤ 1 µg/test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test.
Light Sensitivity: Tandem dye is sensitive to photo-induced oxidation; protect from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.
Excitation/Emission: 488–561 nm / 775 nm; compatible with Blue, Green, Yellow-Green lasers.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for mast cell growth factor (MGF, stem cell factor). It regulates growth and activation of various hematopoietic and non-hematopoietic cells.
Mutations in KIT are linked to gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism.
Activation of c-Kit triggers autophosphorylation and downstream signaling (Ras/ERK, PI3K, PLC-γ, JAK/STAT), essential for hematopoiesis, stem cell maintenance, and gametogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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