Thermo Fisher Scientific PAPPA Monoclonal Antibody (09)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

-

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

-

ELISA (ELISA)

1:8,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

09

Immunogen

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View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.96 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1086780

Product Specific Information

BTE 004-09-02 detects Pregnancy-associated plasma protein A (PAPP-A) from human samples. Heat and SDS denatured PAPP-A is also recognized, but not acid treated PAPP-A.

BTE 004-09-02 has been successfully used in ELISA, Western blotting and Immunohistochemistry procedures. In sandwich ELISA the optimal combination is BTE 004-09-02 used as coating antibody and biotinylated ABS 006-24-02 used as detection antibody. BTE 004-09-02 can also be used as detection antibody in sandwich ELISA in combination with a polyclonal antibody towards PAPP-A in coat. BTE 004-09-02 detect both reduced and unreduced PAPP-A in Western Blotting, though reaction to unreduced is less than that of ABS 006-01-02 and ABS 006-24-02.

The epitope of BTE 004-09-02 is different from ABS 006-01-02 and ABS 006-24-02. The epitope is located on the PAPP-A subunit of the heterotetramer.

NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL

Target Information

Pregnancy-associated plasma protein-A (PAPP-A, Insulin-like growth factor binding protein-4 protease, PAPP-A-proMBP complex) is a large heterottetrameric glycoprotein of approximately 500 kDa, which was first discovered in serum from pregnant women. The hetreotetramer consists of two PAPP-A subunits and two proMBP subunits. Low maternal serum levels of PAPP-A in first trimester biochemical screening is used as a marker of Down`s syndrome (trisomy 21).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.