
Thermo Fisher Scientific Bovine Serum Albumin Polyclonal Antibody, Biotin
Thermo Fisher Scientific의 Biotin 결합 Bovine Serum Albumin Polyclonal Antibody. WB, IHC, ELISA, IP에 적합. Rabbit IgG 기반으로 고순도 정제된 항체. 안정적인 보관 및 높은 특이성 제공.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (IHC) | 1:250–1:1,250 |
| ELISA | 1:1,000–1:5,000 |
| Immunoprecipitation (IP) | 1:500–1:2,500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Albumin (Bovine Serum) |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL PEG, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Bovine serum antibody was purified as an IgG fraction from monospecific antiserum through a multi-step process including delipidation, salt fractionation, and ion-exchange chromatography, followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum, and purified or partially purified Albumin (Bovine Serum). Cross-reactivity against Albumin from other sources may occur but has not been specifically determined.
Target Information
Albumin is a soluble, monomeric protein comprising about one-half of the blood serum protein. It functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein with a molecular weight of approximately 65,000. Synthesized in the liver as preproalbumin, it undergoes cleavage of the N-terminal peptide before release from the rough endoplasmic reticulum. The resulting proalbumin is further processed in the Golgi vesicles to produce the secreted albumin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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