Thermo Fisher Scientific CD47 Monoclonal Antibody (B6H12), PE-Cyanine5, eBioscience

Thermo Fisher Scientific CD47 Monoclonal Antibody (B6H12), PE-Cyanine5, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 700
• APC
• APC-eFluor 780
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Red 700
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The monoclonal antibody B6H12 reacts to CD47, also known as integrin-associated protein (IAP) and neurophilin.
CD47 is a glycosylated five-transmembrane protein with a small, alternatively spliced cytoplasmic domain. It is involved in adhesion through interactions with SIRP (signal regulator protein) and is associated with β3 integrins (CD51/CD61 and CD41/CD61).

This interaction mediates bi-directional signaling to modify neural synaptic activity and regulate macrophage phagocytosis. CD47 acts as a receptor for thrombospondin, and its expression on T cells can mediate activation or apoptosis depending on thrombospondin levels.

Stimulation of CD47 by monoclonal antibody has been shown to induce CD4+CD25- suppressive activity and increase Foxp3 expression.
Expression is found in most hematopoietic cells (T, B cells, monocytes, platelets, erythrocytes) and also in non-hematopoietic cells.
This antibody has reported neutralizing activity.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-diluted and tested by flow cytometry on normal human peripheral blood cells.
Use at 5 µL (0.25 µg) per test in a final volume of 100 µL. Cell number may vary from 10⁵–10⁸ cells/test.

Light Sensitivity: Tandem dye is sensitive to photo-induced oxidation; protect from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.

Excitation: 488–561 nm
Emission: 667 nm
Laser: Blue, Green, Yellow-Green Laser

Target Information

CD47 (Integrin-associated protein, IAP) is a ubiquitously expressed cell surface transmembrane glycoprotein that interacts with several integrins and regulates their functions.
Engagement of CD47 modulates various signaling pathways, including activation of heterotrimeric G proteins and intracellular calcium increase upon cell adhesion.

CD47 is also a receptor for the C-terminal cell binding domain of thrombospondin and may play roles in membrane transport and signal transduction.
It is widely distributed across tissues, reduced on Rh erythrocytes, and acts as a ligand for CD172a (SIRPα), an inhibitory receptor on macrophages that prevents phagocytosis of CD47-positive cells.

The CD47–CD172a system influences cell migration, B cell adhesion, T cell activation, chondrocyte mechanotransduction, and neuronal development, being abundant in synapse-rich brain and retina regions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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