Thermo Fisher Scientific PLCG2 Polyclonal Antibody
PLCG2 단백질을 인식하는 Thermo Fisher Scientific의 토끼 다클론 항체. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용에 적합. 항원 친화 크로마토그래피로 정제되었으며, 동결건조 형태로 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–5 µg/mL |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
| Flow Cytometry (Flow) | 1–3 µg/1×10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived human PLCG2 recombinant protein (Position: R1201–V1258) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 500 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 4 mg trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2746942 |
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
Phosphoinositide-specific phospholipase C (PLC) plays a crucial role in receptor-mediated signal transduction through the generation of inositol 1,4,5-triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate.
Mammalian PLC isozymes include PLCβ1, PLCβ2, PLCβ3, PLCβ4, PLCγ1, PLCγ2, PLCδ1, PLCδ2, and PLCε.
PLCδ1 acts as a calcium signal amplifier and is activated by an atypical GTP-binding protein. It functions as an effector for transglutaminase II-mediated oxytocin receptor and α1B-adrenoreceptor signaling.
Mouse PLCδ1 is highly expressed in brain, heart, lung, and testis, and is abnormally accumulated in Alzheimer’s disease brains, suggesting a role in AD pathology.
PLCδ2 is markedly expressed in type II intestinal metaplasia and adenocarcinoma. Its expression in type I intestinal metaplasia is generally benign but may evolve toward neoplastic transformation, making PLCδ2 a potential marker for gastric malignant transformation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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