Thermo Fisher Scientific CD137 (4-1BB) Monoclonal Antibody (17B5), Functional Grade, eBioscience

Thermo Fisher Scientific CD137 (4-1BB) Monoclonal Antibody (17B5), Functional Grade

Applications and Tested Dilution

Product Specifications

Available Formats:

• APC
• Biotin
• eFluor 450
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The 17B5 monoclonal antibody reacts with mouse CD137 (4-1BB), a TNFR superfamily member expressed on activated T cells (day 4–6 post TCR activation). CD137 interaction with 4-1BBL delivers a costimulatory signal critical for T cell–APC interaction. Recent data suggest CD137 expression on Tregs may regulate their proliferation.
The 17B5 antibody exhibits strong antagonistic effects in in vitro functional assays.

Applications Reported: Flow cytometric analysis and functional assays.
Applications Tested: Tested on activated mouse splenocytes; ≤0.5 µg/test (100 µL final volume). Cell number should be optimized (10⁵–10⁸ cells/test). Antibody titration recommended for best performance.

Storage and Handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: >90% (SDS-PAGE).
Endotoxin Level: <0.001 ng/µg antibody (LAL assay).
Aggregation: <10% (HPLC).

Target Information

CD137 (TNFRSF9, 4-1BB) is an inducible costimulatory molecule mainly expressed on activated T cells. Its ligand, 4-1BBL, is found on activated macrophages, mature B cells, hematopoietic stem cells, and myeloid progenitor cells.
CD137 signaling supports survival and clonal expansion of activated and memory T cells, while suppressing myelopoiesis and dendritic cell development. Activation induces cytokine release that regulates monocyte survival. Soluble CD137 may provide negative feedback for immune responses.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.