Thermo Fisher Scientific CD134 (OX40) Monoclonal Antibody (OX-86), Functional Grade, eBioscience

Thermo Fisher Scientific CD134 (OX40) Monoclonal Antibody (OX-86), Functional Grade, eBioscience

Applications and Tested Dilution

Product Specifications

Available Conjugates

• Unconjugated
• APC
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 780
• Request custom conjugation

Product Specific Information

The OX-86 monoclonal antibody reacts with mouse CD134 (OX40), a 50 kDa type I membrane glycoprotein of the TNF receptor superfamily expressed by activated mouse T lymphocytes. Mouse CD134 is expressed by both activated CD4+ and CD8+ T cells. Interaction with CD252 (OX40 ligand) influences T cell-dependent humoral responses, primary T cell expansion, survival, memory T cell pool size, and tolerance regulation in CD4+ T cells.

Applications Reported:
The OX-86 antibody is suitable for flow cytometric analysis and functional assays. It does not block CD134 ligand binding.

Applications Tested:
Tested by flow cytometric analysis of activated mouse splenocytes. Recommended ≤ 0.5 µg per test (100 µL final volume). Optimize antibody titration for best assay performance.

Storage and Handling:
Use in sterile environment.

Filtration:
0.2 µm post-manufacturing filtered.

Purity:

90%, determined by SDS-PAGE.

Endotoxin Level:
<0.001 ng/µg antibody (LAL assay).

Aggregation:
<10%, determined by HPLC.

Target Information

OX40 is a receptor on T cells that promotes proliferation and survival upon activation, enhancing immune responses against cancer and infections. OX40 agonists show promise in immunotherapy, potentially improving outcomes with chemotherapy and checkpoint inhibitors, and may have applications in autoimmune and allergic conditions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.