Thermo Fisher Scientific aminoxyTMTsixplex Label Reagent Set, 1 x 0.2 mg, 1 x 0.2 mg (per tag)

Thermo Scientific™ aminoxyTMTsixplex™ Label Reagent Set

The aminoxyTMTsixplex™ Label Reagents share an identical structure but contain different numbers of ¹³C and ¹⁵N isotopes in the mass reporter. The optimized labeling procedure can be completed in one hour. After tag labeling, samples are quenched and cleaned using HILIC solid-phase extraction before mass spectrometry analysis.

The six compounds of the aminoxyTMTsixplex™ Reagent Set have the same mass (isobaric) and chemical structure (carbonyl-reactive aminoxy group, spacer arm, and mass reporter). The specific distribution of ¹³C and ¹⁵N isotopes results in unique reporter masses (126–131 Da) in the low-mass region, enabling relative quantitation of labeled molecules in multiplexed MS samples representing six different conditions.

For glycobiology MS applications, these reagents enable quantitative profiling of glycan isoforms and biomarker discovery. They improve ionization and retention of labeled glycans by reversed-phase liquid chromatography (RPLC). The aminoxyTMT reagents can quantify carbohydrates, steroids, or oxidized proteins.

Features

• Quantitative — enables relative quantitation of glycans or other carbonyl-containing biomolecules from multiple samples
• Stable — oxime bond and products are stable, no reduction step required
• Efficient — >90% labeling efficiency in one hour
• Sensitive — 20-fold increase in signal-to-noise ratio compared to unlabeled glycans
• Multiplex — analyze up to six samples concurrently
• Optimized — reagents and protocol optimized for high labeling efficiency and recovery

Includes

• Sufficient aminoxyTMT tags for five sixplex experiments

Requires

• PNGase F (or PNGase A) glycosidase
• Waters Oasis™ HLB columns

Applications

• Multiplex up to 6 samples in one experiment
• Relative quantitation of glycans
• Study structural diversity of protein glycosylation
• Investigate glycosylation in cell signaling and regulation
• Cancer biomarker discovery and biotherapeutics analysis

Native glycans are difficult to study by mass spectrometry due to poor ionization efficiency. The aminoxy group shows better reactivity with carbonyls and improved stability compared to hydrazides. Labeling with aminoxyTMT reagents enhances ionization and sensitivity, enabling reliable quantitation of glycans across multiple samples.

These products are subject to a limited use label license.

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