Thermo Scientific™

Pierce™ S-Nitrosylation Western Blot Kit and HENS Buffer

Thermo Scientific Pierce S-Nitrosylation Western Blot Kit enables sensitive detection of protein S-nitrosocysteine post-translational modifications in a complete, easy-to-use kit or stand-alone HENS buffer for S-Nitrosylation Western Blot Kit.

The Pierce S-Nitrosylation Western Blot Kit provides cell lysis/reaction buffer, sulfhydryl-reactive blocking agent, reducing agent, labeling agent, and anti-TMT antibody to label 40 samples (100 μg each). As with traditional S-nitrosylation switch assays, unmodified cysteines are blocked by a sulfhydryl-reactive compound. S-nitrosylated cysteines are selectively reduced with ascorbate in HENS Buffer for labeling with iodoTMTzero reagents, which irreversibly bind to the cysteine thiol that was S-nitrosylated. Detection of the TMT reagent-modified proteins is facilitated using an anti-TMT antibody.

사양

설명Pierce™ S-Nitrosylation Western Blot Kit

검출 방법S-Nitrosylation

제품라인Pierce™

수량40 Reactions

반응성All species

Unit SizeEach