Thermo Fisher Scientific Ly-6C Monoclonal Antibody (HK1.4), APC-eFluor 780, eBioscience

Thermo Fisher Scientific Ly-6C Monoclonal Antibody (HK1.4), APC-eFluor 780, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.5 µg/test
Publications: View 75 publications

Product Specifications

Additional Formats

• Alexa Fluor 488
• APC
• eFluor 450
• Functional Grade
• PE
• PE-Cyanine7
• PerCP-Cyanine5.5
• Super Bright 436
• Request custom conjugation

Product Specific Information

The monoclonal antibody HK1.4 recognizes mouse Ly-6C, a GPI-linked protein of the Ly6 family.
Ly-6C is found on monocytes/macrophages, endothelial cells, granulocytes, and subsets of lymphocytes.
Expression varies across mouse strains:

• Ly6C.1 (C57Bl/6, SJL): stains CD4 and CD8 cells
• Ly6C.2 (BALB/c, 3H/He): stains CD8 cells only

In vitro, HK1.4 can increase proliferation and cytokine release.

Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of mouse splenocytes (≤ 0.5 µg/test, 100 µL final volume)

APC-eFluor® 780 emits at 780 nm, excited by the Red laser (633 nm).
Ensure instrument compatibility with this fluorochrome.

Light Sensitivity: Sensitive to photo-induced oxidation — protect from light.
Fixation: Samples may be stored in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) for up to 3 days at 4°C in the dark.
Excitation: 633–647 nm
Emission: 780 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

Granulocytes are white blood cells containing granules in their cytoplasm.
They are also known as polymorphonuclear leukocytes (PMN or PML) due to the lobed nucleus.
Neutrophil granulocytes are the most abundant type.
Granulocytes are released from bone marrow under complement protein regulation.

For Research Use Only. Not for use in diagnostic procedures.
Not for resale without express authorization.

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