
Thermo Fisher Scientific F(ab`)2-Goat anti-Mouse IgG (H+L) Secondary Antibody, Biotin
Biotin으로 표지된 F(ab`)2 형태의 Goat anti-Mouse IgG (H+L) 2차항체로, Western blot, ICC/IF, Flow cytometry, ELISA 등 다양한 응용에 적합합니다. 높은 특이성과 감도를 보장하며, Affinity Chromatography로 정제되었습니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:20,000 |
| Flow Cytometry (Flow) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535985 |
| Target | IgG |
| Antibody Form | F(ab`)2 Fragment |
Product Specific Information
Each lot of antibody is tested for sensitivity using ELISA and for specificity using immunoelectrophoresis (IEP).
Target Information
Anti-Mouse secondary antibodies are affinity-purified with well-characterized specificity for mouse immunoglobulins. They are useful for detection, sorting, or purification of target molecules. Secondary antibodies increase versatility by enabling multiple detection systems such as HRP, AP, or fluorescence, and enhance sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. These antibodies are typically generated by immunizing the host animal with pooled mouse immunoglobulins and further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to produce highly specific reagents.
Additional Formats Available
다양한 conjugate 형식으로 제공됩니다:
- Agarose
- Alexa Fluor 488, 555, 647, 680, 790
- Alexa Fluor Plus 405, 488, 647
- DyLight 350, 405, 488, 550, 594, 650, 680, 800
- FITC, TRITC, Rhodamine
- HRP, AP, APC, PE, PerCP-eFluor 710
- Qdot 525–800, WesternDot 625
- eFluor 570, 660
- NovaFluor Red, Yellow series
- Functional Grade 및 맞춤형 conjugation 가능
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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