Invitrogen™

AccuPrime™ Pfx DNA Polymerase

AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis.

AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading (3→5 exonuclease) activity. Platinum anti-Pfx DNA polymerase antibodies inhibit polymerase activity, providing hot-start capabilities for improved PCR specificity (see figure). Thermostable AccuPrime accessory proteins enhance specific primer-template hybridization during every cycle of PCR, increasing specificity, yield, and robustness over Platinum Pfx alone.

사양

검출 방법Primer-probe

GC-Rich PCR PerformanceLow

중합효소AccuPrime Pfx DNA Polymerase

반응 속도Standard

수량200 Reactions

배송 조건Wet or Dry Ice

용도(애플리케이션)Hot-start PCR, High-fidelity PCR

Fidelity (Taq 대비)26X

핫 스타트Built-In Hot Start

반응 수200 Reactions

오버행Blunt

반응 형식Separate Components

크기(최종 제품)12 kb or less

부피100 μL

Unit SizeEach