Thermo Fisher Scientific SARS-CoV-2 Spike Protein (S1/S2) Recombinant Human Monoclonal Antibody (bcb03)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:4,000-1:5,000

View 1 publication 1 publication

ELISA (ELISA)

1:80,000

-

Neutralization (Neu)

Assay-dependent

-

Product Specifications

Published species

Virus

Host/Isotype

Human / IgG

Class

Recombinant Monoclonal

Type

Antibody

Clone

bcb03

Immunogen

Fully humanized monoclonal antibody paired with human monoclonal antibody corresponding to SARS-CoV-2 Spike Protein (S-ECD/RBD)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2866562

Product Specific Information

Purity: Greater than 95% as determined by reducing SDS-PAGE

Target: Specificity for Spike protein regions S1/S2, RBD and ECD.

Target Information

SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold symptoms to severe effects including death and respiratory distress. The CoV Spike (S) protein plays the most important roles in viral attachment, fusion and entry, and serves as a target for development of antibodies, entry inhibitors and vaccines. Of note is the Spike protein receptor-binding domain (RBD, S-RBD) in SARS-CoV-2 Spike protein which binds strongly to human and bat angiotensin-converting enzyme 2 (ACE2) receptors. SARS-CoV-2 RBD exhibits significantly higher binding affinity to ACE2 receptor than SARS-CoV RBD in comparison. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells. SARS-CoV-2 S is 1273 amino acids long and consists of a signal peptide (amino acids 1-13) located at the N-terminus, the S1 subunit (14-685 residues), and the S2 subunit (686-1273 residues); the last two regions are responsible for receptor binding and membrane fusion, respectively. The S-ECD is the extracellular domain of the Spike protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.