
Norgen West Nile Virus (WNV) TaqMan RT-PCR Detection Kits
West Nile Virus(WNV) 검출용 TaqMan RT-PCR 키트로 고감도 RNA 바이러스 진단 가능. CE-IVD 인증 버전 제공. 프라이머·프로브·양성·음성 대조군 포함. 분자 수준에서 빠르고 정확한 WNV 검출에 적합.
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Norgen West Nile Virus (WNV) TaqMan RT-PCR Detection Kits
SKU: TM44210
Features and Benefits
- Detection kits for West Nile Virus (WNV)
- CE-IVD marked version available for in vitro diagnostic use
- Available in TaqMan format for analysis
Product Description
West Nile Virus (WNV) belongs to the RNA virus family Flaviviridae and is primarily transmitted through arthropod vectors such as mosquitoes. The virus mainly infects birds but can also infect humans and pets such as cats and dogs.
Most human infections are asymptomatic; however, some cases progress to a febrile stage with flu-like symptoms known as West Nile Fever. Severe infections can become neuroinvasive, causing meningitis or encephalitis, which may be fatal.
Because WNV symptoms resemble other common diseases, early molecular-level detection is crucial for accurate diagnosis.
Product Variants
WNV TaqMan RT-PCR Kit (100 reactions)
- Ready-to-use format including Master Mix for target and PCR control
- Specific Primer and Probe mix for the pathogen/virus/viroid of interest
- Includes Positive and Negative Controls to confirm reagent integrity
WNV TaqMan RT-PCR Probe/Primer Set and Controls (100 reactions)
- Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
- Includes Nuclease-free water
- Compatible with Norgen’s RT-PCR Master Mix (#28113) or user-supplied master mix
Supporting Data
TaqMan PCR Kit - Figure 1
Example of TaqMan One-step RT-PCR positive result.
Both PCR signals above the baseline from FAM and HEX channels indicate successful amplification.
그래프 정보 (OCR 추출):
두 그래프 모두 “Amplification” 제목을 가지며,
- 상단 그래프: FAM channel (파란색)
- 하단 그래프: HEX channel (초록색)
- X축: Cycles
- Y축: RFU
TaqMan PCR Kit - Figure 2
Example of TaqMan One-step RT-PCR negative result.
No target RNA detected in FAM channel, but amplification signal from HEX indicates successful PCR.
그래프 정보 (OCR 추출):
- 상단 그래프: Amplification (FAM channel)
- X축: Cycles (0~40), Y축: RFU (-100~600)
- 하단 그래프: Amplification (HEX channel)
- X축: Cycles (0~40), Y축: RFU (-100~900)
TaqMan PCR Kit - Figure 3
Example of TaqMan One-step RT-PCR inhibition result.
No signal detected from both FAM and HEX channels. It is recommended to repeat sample preparation using the recommended RNA purification kit.
그래프 정보 (OCR 추출):
두 그래프 모두 “Amplification” 제목을 가지며,
- 상단: FAM channel
- 하단: HEX channel
- X축: Cycles (10~40), Y축: RFU
Storage Conditions and Product Stability
All kit components can be stored for 1 year after production without performance reduction.
Store all components at -20°C upon arrival.
| Component | Cat. TM44250 (100 preps) | Cat. TM44210 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 × 700 μL | - |
| WNV Primer & Probe Mix | 280 μL | 280 μL |
| WNV Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
Documentation
Protocols
- (TM44250) WNV TaqMan RT-PCR Kit - Protocol (100 rxns)
- (TM44210) WNV TaqMan Probe/Primer and Control Set - Protocol (100 rxns)
Safety Data Sheets
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