Thermo Fisher Scientific Phospho-KAP-1 (Ser824) Polyclonal Antibody

Applications

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:100–1:500

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• Based on 100% sequence identity, predicted to react with Mouse, Orangutan, Monkey, Gorilla, Chimpanzee, Northern white-cheeked gibbon, Crab-eating macaque, and White-tufted-ear marmoset

Target Information

The KRAB (Kruppel-associated box) domain is approximately 45 amino acids in length and acts as a transcriptional repression domain found in numerous transcription factors. Over 220 KRAB-zinc finger protein (KRAB-ZFP) genes have been identified in the human genome. These proteins repress transcription via interactions with KAP-1 (KRAB-associated protein 1), an 835-amino-acid polypeptide containing a RING finger, B boxes, and a PHD finger.
KAP-1 forms complexes with KRAB-domain transcription factors to enhance repression efficiency and interacts directly with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel-associated box-containing zinc finger protein 1). It targets KRAZ1 to centromeric heterochromatin regions containing HP1α, contributing to transcriptional repression regulation. Mutant KAP-1 variants that bind KRAB but not HP1 cause random KRAZ1 distribution and strong transcriptional activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.