Thermo Fisher Scientific eIF3B/EIF3S9 Polyclonal Antibody
인간 eIF3B 단백질을 표적으로 하는 Thermo Fisher Scientific의 다클론 항체. Western blot, IHC, IP 등 다양한 응용 가능. 항원 친화 크로마토그래피로 정제된 액상 제품이며 4°C에서 보관. 단백질 합성 개시 복합체 연구에 적합.
- 카탈로그번호
- A301761A
- 판매단위
- pk
카탈로그
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Thermo Fisher Scientific eIF3B/EIF3S9 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 100 and 150 of human eukaryotic translation initiation factor 3, subunit B (EIF3B/EIF3S9) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). It stimulates mRNA recruitment and scanning for AUG recognition, and is involved in disassembly and recycling of post-termination ribosomal complexes. The complex also regulates translation of mRNAs involved in cell proliferation, differentiation, and apoptosis.
[UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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