Thermo Fisher Scientific DTYMK Polyclonal Antibody
Rabbit polyclonal antibody against human DTYMK for WB and IHC applications. Affinity-purified with >95% purity. Recognizes amino acids 3–48 of DTYMK. Supplied as liquid form, 1 mg/mL, stored at 4°C or -20°C. For research use only.
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Thermo Fisher Scientific DTYMK Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 3–48 of Human DTYMK |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2719233 |
Product Specific Information
The antibody was affinity-purified from rabbit antiserum by affinity chromatography using an epitope-specific immunogen. The purity is >95% as verified by SDS-PAGE.
Target Information
Thymidylate kinase (DTYMK) catalyzes the phosphorylation of dTMP to form dTDP, a key step in the dTTP synthesis pathway required for DNA synthesis. DTYMK is essential for DNA synthesis and serves as an intermediate enzyme in the pathway of several pyrimidine analog drugs, including 5-Fluorouracil (5-FU), a chemotherapeutic agent used for solid tumors. The human DTYMK gene was identified through functional complementation of a Saccharomyces cerevisiae cell cycle mutant (cdc8). DTYMK mRNA levels and enzyme activities are regulated by cell cycle and growth pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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