Invitrogen™

EnzChek™ Phosphatase Assay Kit

Using the EnzChek Phosphatase Assay Kit continuous monitoring of phosphatase activity, even at neutral or acidic pH, can be achieved.자세히 알아보기

Using the EnzChek Phosphatase Assay Kit continuous monitoring of phosphatase activity, even at neutral or acidic pH, can be achieved. The kit contains Molecular Probes` patented 6,8-difluoro-4-methylumbelliferyl phosphate (DiFMUP) substrate (D6567). DiFMUP is about 100 times more sensitive than 4-methylumbelliferyl phosphate (MUP) for the detection of prostatic acid phosphatase at pH 5.5. The kit is perfect for the continuous assay of prostatic acid phosphatase, protein phosphatase 1 or almost any other phosphatase that can be assayed with nonprotein-based substrates such as MUP or 4-nitrophenyl phosphate (PNPP). Each kit contains a sufficient amount of substrate for ∼1000 assays, using a reaction volume of 100 μL per assay. Fluorescence (excitation/emission maxima ∼358/455 nm) can be measured in a fluorescence microplate reader or a standard fluorometer.

사양

색상Blue

검출 방법Fluorescence

용도(애플리케이션)Phosphatase Assay

용도 (장비)Microplate Reader

제품 유형Phosphatase Assay

염료 유형DiFMU

방출360⁄460

제품라인EnzChek™

수량1000 Assays

배송 조건Room Temperature

기질DiFMUP

기질 특성Chemical Substrate

기질 유형Phosphatase Substrate

타겟 효소Phosphatases

Unit SizeEach