Thermo Fisher Scientific NGEF Polyclonal Antibody
NGEF 단백질을 인식하는 Rabbit Polyclonal Antibody로, Human, Mouse, Rat에 반응합니다. Western blot, ICC/IF, ELISA에 적합하며, 고순도의 Affinity Chromatography 정제 제품입니다. 액상 형태로 -20°C에서 보관하며 연구용으로 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 429–710 of human NGEF (NP_062824.2) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2855439 |
Product Specific Information
Immunogen sequence:
RVEERSEREC TALDAHKELE MVVKACNEGV RKMSRTEQMI SIQKKMEFKI KSVPIISHSR WLLKQGELQQ MSGPKTSRTL RTKKLFHEIY LFLFNDLLVI CRQIPGDKYQ VFDSAPRGLL RVEELEDQGQ TLANVFILRL LENADDREAT YMLKASSQSE MKRWMTSLAP NRRTKFVSFT SRLLDCPQVQ CVHPYVAQQP DELTLELADI LNILDKTDDG WIFGERLHDQ ERGWFPSSMT EEILNPKIRS QNLKECFRVH KMDDPQRSQN KDRRKLGSRN RQ
Target Information
Acts as a guanine nucleotide exchange factor (GEF) that differentially activates the GTPases RHOA, RAC1, and CDC42. Plays a role in axon guidance by regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA, resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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