Thermo Fisher Scientific NGEF Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunocytochemistry (ICC/IF)

1:50-1:100

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 429-710 of human NGEF (NP_062824.2). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NGEF,uniProtId:Q8N5V2-1,ncbiNodeId:9606,antigenRange:429-710,antigenLength:710,antigenImageFileName:PA5-110028_NGEF_Q8N5V2-1_Rabbit.svg,antigenImageFileNamePDP:PA5-110028_NGEF_Q8N5V2-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2855439

Product Specific Information

Immunogen sequence: RVEERSEREC TALDAHKELE MVVKACNEGV RKMSRTEQMI SIQKKMEFKI KSVPIISHSR WLLKQGELQQ MSGPKTSRTL RTKKLFHEIY LFLFNDLLVI CRQIPGDKYQ VFDSAPRGLL RVEELEDQGQ TLANVFILRL LENADDREAT YMLKASSQSE MKRWMTSLAP NRRTKFVSFT SRLLDCPQVQ CVHPYVAQQP DELTLELADI LNILDKTDDG WIFGERLHDQ ERGWFPSSMT EEILNPKIRS QNLKECFRVH KMDDPQRSQN KDRRKLGSRN RQ

Target Information

Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.