Thermo Fisher Scientific SHISA4 Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:50

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human SHISA4. Recombinant protein control fragment (Product #RP-101607). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SHISA4,uniProtId:Q96DD7-1,ncbiNodeId:9606,antigenRange:30-79,antigenLength:197,antigenImageFileName:PA5-63939_SHISA4_Q96DD7-1_Rabbit.svg,antigenImageFileNamePDP:PA5-63939_SHISA4_Q96DD7-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.05 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2647274

Product Specific Information

Immunogen sequence: DCLWYLDRNG SWHPGFNCEF FTFCCGTCYH RYCCRDLTLL ITERQQKHCL

Highest antigen sequence identity to the following orthologs: Mouse - 96%, Rat - 96%.

Target Information

SHISA4 plays an essential role in the maturation of presomitic mesoderm cells by individual attenuation of both FGF and WNT signaling. The Shisa family of single-transmembrane proteins is characterized by an N-terminal cysteine-rich domain and a proline-rich C-terminal region. Its founding member, Xenopus Shisa, promotes head development by antagonizing Wnt and FGF signaling. Shisa physically interacted with immature forms of the Wnt receptor Frizzled and the FGF receptor within the ER and inhibited their posttranslational maturation and trafficking to the cell surface. Loss of Shisa function sensitized the neuroectoderm to Wnt signaling and suppressed head formation during gastrulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.