Thermo Fisher Scientific Donkey anti-Sheep IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 680

Applications

Tested Dilution

Western Blot (WB)

0.04-1.0 µg/mL

Immunocytochemistry (ICC/IF)

1-10 µg/mL

Product Specifications

Species Reactivity

Sheep

Host/Isotype

Donkey / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Gamma Immunoglobins Heavy and Light chains

Conjugate

Alexa Fluor™ 680 Alexa Fluor™ 680 Alexa Fluor™ 680

View additional formats

• [Alexa Fluor 350 ](/antibody/secondary/product-name/Sheep IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 350/host/Donkey/)
• [Alexa Fluor 488 ](/antibody/secondary/product-name/Sheep IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 488/host/Donkey/)
• [Alexa Fluor 546 ](/antibody/secondary/product-name/Sheep IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 546/host/Donkey/)
• [Alexa Fluor 555 ](/antibody/secondary/product-name/Sheep IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 555/host/Donkey/)
• [Alexa Fluor 568 ](/antibody/secondary/product-name/Sheep IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 568/host/Donkey/)
• [Alexa Fluor 594 ](/antibody/secondary/product-name/Sheep IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 594/host/Donkey/)
• [Alexa Fluor 633 ](/antibody/secondary/product-name/Sheep IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 633/host/Donkey/)
• [Alexa Fluor 647 ](/antibody/secondary/product-name/Sheep IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 647/host/Donkey/)
• Request custom conjugation

Excitation/Emission Max

681/704 nm View spectra

Form

Liquid

Concentration

2 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.5

Contains

5mM sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient

RRID

AB_2535755

Target

IgG

Cross Adsorption

Against mouse, rabbit, bovine and human sera and human IgG

Antibody Form

Whole Antibody

Product Specific Information

This secondary antibody is designed for fluorescent Western blot detection on various near-infrared fluorescence instruments. This antibody can be used for multi-color and multiplexing detection when using other antibodies conjugated to compatible Alexa Fluor™ dyes and wavelengths. Other applications of this antibody include immunofluorescent and fluorescent imaging applications when using instrumentation with appropriate excitation and detection capabilities.

Product will be shipped at Room Temperature.

Target Information

Anti-Sheep secondary antibodies are affinity-purified antibodies with well-characterized specificity for ovine (sheep) immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.