Thermo Fisher Scientific IDH3B Polyclonal Antibody

Applications

• Western Blot (WB): 0.3–1 µg/mL

Product Specifications

Product Specific Information

• This antibody is expected to recognize isoform a (NP_008830.2) and isoform b (NP_777280.1).
• Tested in Peptide ELISA: antibody detection limit dilution 1:1,000.

Target Information

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses: one utilizes NAD(+) and the other NADP(+) as electron acceptors.
Five isocitrate dehydrogenases have been reported:

• Three NAD(+)-dependent enzymes localized to the mitochondrial matrix
• Two NADP(+)-dependent enzymes (one mitochondrial, one cytosolic)

NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated, rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer composed of two alpha subunits, one beta subunit, and one gamma subunit.
The protein encoded by this gene is the beta subunit of one NAD(+)-dependent isocitrate dehydrogenase isozyme. Three alternatively spliced transcript variants encoding different isoforms have been described for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.