Thermo Fisher Scientific E-selectin Monoclonal Antibody (ENA2)
E-selectin(CD62E)에 특이적으로 반응하는 ENA2 단클론 항체로, 인간 활성화 내피세포를 인식하며 염증 관련 연구에 적합합니다. IHC, ICC/IF, 기능 및 억제 분석에 사용 가능하며, Protein G로 정제된 액상 항체입니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Frozen) (IHC (F)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| Functional Assay (Functional) | 1:50 |
| Inhibition Assays (IA) | 1:50 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ENA2 |
| Immunogen | ENA2 reacts with E-selectin CD62-E |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 100 µg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 0.1% BSA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
| RRID | AB_3091106 |
Product Specific Information
ENA2 reacts with E-selectin (CD62-E), previously designated as Endothelial Leucocyte Adhesion Molecule-1 (ELAM-1).
The antibody reacts with human endothelial cells activated with TNF-alpha, IL-1, or endotoxin.
It also reacts with cells transfected with the E-selectin gene and inhibits the adhesion of granulocytes, both neutrophilic and eosinophilic.
Target Information
Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes.
Leukocyte adhesion also occurs in capillaries and small venules after inflammation onset.
This response involves a cascade of adhesion receptors, including selectins and members of the immunoglobulin superfamily.
E-selectin (CD62E) is an endothelial cell-specific selectin expressed only after activation with proinflammatory cytokines.
IL-1, TNF-alpha, and bacterial components such as lipopolysaccharides induce E-selectin transcription through an NFkB-dependent signaling cascade.
E-selectin is associated with blood vessel endothelium in diverse inflammatory conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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