Thermo Fisher Scientific EGFR L858R Monoclonal Antibody (UMAB234), UltraMAB

Applications

• Immunohistochemistry (Paraffin) (IHC (P)): 1:300

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

EGFR (Epidermal Growth Factor Receptor, HER1, ErbB1) is encoded by the EGFR gene on chromosome 7 in humans.
It belongs to the HER/ErbB family of receptor tyrosine kinases (ERbB2, ERbB3, ERbB4).
EGFR is a transmembrane receptor that, upon ligand binding (e.g., EGF, TGF-α), undergoes dimerization and autophosphorylation, activating downstream signaling pathways such as Ras-ERK, PI3K-Akt, Jak-STAT, and PKC.
Phosphorylation at Y1086 enables GRB2 binding, activating the MAPK pathway.
EGFR activation results in receptor endocytosis and either degradation or recycling.
Mutations in EGFR are associated with lung cancer, and overexpression correlates with poor prognosis in various tumors (head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, ovary, etc.).
This antibody specifically recognizes EGFR mutant L858R.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.