Thermo Fisher Scientific CHIA Polyclonal Antibody
Rabbit polyclonal antibody targeting human CHIA protein. Validated for WB, IHC, and ICC/IF applications. High purity (>95%) and affinity-purified. Suitable for research on Th2-mediated immune responses and asthma mechanisms.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant full-length Human CHIA (UniProt ID: Q9BZP6-1, antigen range: 1–476, length: 476) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2719823 |
Product Specific Information
The antibody was affinity-purified from rabbit antiserum using epitope-specific immunogen. Purity >95% as confirmed by SDS-PAGE.
Target Information
CHIA degrades chitin and chitotriose, potentially involved in defense against nematodes and other pathogens. Three isoforms are produced by alternative splicing. Expression is induced via a Th2-specific, interleukin-13-mediated pathway in epithelial cells and macrophages. CHIA may play a key role in IL-13-induced responses in Th2-dominated disorders such as asthma. It hydrolyzes N-acetyl-beta-D-glucosaminide 1,4-beta-linkages in chitin and chitodextrins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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