Thermo Fisher Scientific Cytochrome C Antibody Cocktail

Applications

Tested Dilution

Publications

Western Blot (WB)

3.6 µg/mL

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / Ig

Class

Cocktail

Type

Antibody

Clone

Cocktail

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.9 mg/mL

Purification

IgG fraction

Storage buffer

HEPES buffered saline

Contains

0.02% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Wet ice

RRID

AB_2532229

Product Specific Information

ApoTrack™ Cytochrome c WB Cocktail (Sku# 459160) provides a means to detect apoptosis while detecting release of cytochrome c, as well as a cytoplasmic and mitochondrial markers with the help of western blot. This cocktail provides a means to detect cytochrome c, as well as a cytoplasmic and mitochondrial markers

Antibodies Cocktail Contains:

Mouse anti Cyt. c monoclonal:

Amount: 50 µg

Working concentration: 1 µg/mL

Mouse anti GAPDH monclonal:

Amount: 5 µg

Working concentration: 0.1 µg/mL

Mouse anti PDH-E1-alpha monoclonal:

Amount: 100 µg

Working concentration: 2 µg/mL

Mouse anti C-V-alpha monoclonal:

Amount: 25 µg

Working concentration: 0.5 µg/mL

Target Information

Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.