Thermo Fisher Scientific NNT Monoclonal Antibody (8B4BB10)
NNT 단백질을 인식하는 Mouse monoclonal antibody로 Western blot, IHC, ICC, Flow cytometry, IP에 사용 가능. 다양한 종(Bovine, Human, Mouse, Rat)에 반응하며, 4°C에서 보관. 연구용으로 NADPH 생성 관련 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5 µg/mL | View 1 publication |
| Immunohistochemistry (IHC) | 1:100 | - |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | - |
| Flow Cytometry (Flow) | 1 µg/mL | - |
| Immunoprecipitation (IP) | Assay-dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 8B4BB10 |
| Immunogen | Human heart mitochondria (target family: NNT, UniProt ID: Q13423-1, antigen range: 1–1086) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | HEPES buffered saline |
| Contains | 0.02% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Wet ice |
| RRID | AB_10375040 |
Product Specific Information
Positive control samples include:
- Normal Human skin fibroblasts (strain HDFn)
- Human cerebellum tissue
- Mitochondria from Human liver, Bovine heart, Rat liver, and Mouse liver
- HepG2 and HL-60 cells
Target Information
This gene encodes an integral protein of the inner mitochondrial membrane. The enzyme couples hydride transfer between NAD(H) and NADP(+) to proton translocation across the inner mitochondrial membrane. Under most physiological conditions, the enzyme uses energy from the mitochondrial proton gradient to produce high concentrations of NADPH, which is essential for biosynthesis and free radical detoxification.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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