Thermo Fisher Scientific H1FOO Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:1,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human H1FOO. Recombinant protein control fragment (Product #RP-96499).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.10 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2642288

Product Specific Information

Immunogen sequence: YRKTKAESKS SKPTASKVKN GAASPTKKKV VAKAKAPKAG QGPNTKAAAP AKGSGSKVVP AHLSRKTEAP KGPRKAGLPI KASSSKVSSQ

Highest antigen sequence identity to the following orthologs: Mouse - 33%, Rat - 36%.

Target Information

Eukaryotic Histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fibers. Two molecules of each of the four core Histones (Histone H2A, H2B, H3, and H4) form the octamer, which consists of two H2A-H2B dimers and two H3-H4 dimers that are nearly symmetrical by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. H1FOO (H1 histone family, member O, oocyte-specific), also known as OSH1, is a 346 amino acid oocyte-specific Histone that localizes to both the nucleus and the cytoplasm. Expressed as multiple alternatively spliced isoforms, H1FOO is thought to play an important role in gene control during oogenesis and early embryogenesis and is crucial for meiotic maturation of germinal vesicle-stage oocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.