Thermo Fisher Scientific Phospho Enol Pyruvate Carboxylase Polyclonal Antibody, Biotin
식물 유래 Phospho Enol Pyruvate Carboxylase를 인식하는 Biotin 결합 폴리클로날 항체. Western blot, ELISA, IP에 적합하며 고순도 정제. 광의존적 인산화 연구 및 광합성 관련 효소 분석에 유용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| ELISA | 1:5,000–1:20,000 |
| Immunoprecipitation (IP) | 1:500–1:2,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Plant |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Phospho-enol-pyruvate Carboxylase [Maize Leaves] |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid freeze/thaw cycles. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Anti-Phospho Enol Pyruvate Carboxylase antibody is an IgG fraction purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation, and ion exchange chromatography, followed by extensive dialysis against the specified buffer.
Immunoelectrophoresis assay resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum, and purified Phospho-enol-Pyruvate-Carboxylase [Maize]. Cross-reactivity with other sources may occur but has not been determined.
Target Information
Phosphoenolpyruvate Carboxylase catalyzes the carboxylation of phosphoenolpyruvate (PEP) to form oxaloacetate, a four-carbon dicarboxylic acid involved in the tricarboxylic acid cycle.
This enzyme is regulated by light-reversible phosphorylation and participates in the C3 acid pathway as part of photosynthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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