Thermo Fisher Scientific ALKBH2 Polyclonal Antibody

Applications

Immunocytochemistry (ICC/IF)

• Tested Dilution: 0.25–2 µg/mL

Product Specifications

Product Specific Information

Immunogen sequence:
LVKGAQGGLLR KQEEQEPTGE EPAVLGGDKE STRKRPRREA PGNGGHSAGP SWRHIRAEGL DCSYTVLFGK AE

• Highest antigen sequence identity to orthologs: mouse 37%, rat 40%

Target Information

The E. coli AlkB protein protects against the cytotoxicity of methylating agents by repairing specific DNA lesions generated in single-stranded DNA. ALKBH2 and ALKBH3 are mammalian homologs of AlkB that catalyze the removal of 1-methyladenine and 3-methylcytosine, modifications that, if left unchecked, could lead to cancerous cells.
Mutations in both ALKBH2 and ALKBH3 have been observed in pediatric brain tumors, indicating that these proteins are important in the prevention of cancer formation.
Like the histone demethylase JMJD1A, ALKBH2 is a non-heme iron enzyme inhibited by Nickel ions, suggesting that inhibition of ALKBH2 by Nickel ions may play a role in cancer development.
Conversely, ALKBH2 mRNA and protein levels are increased in glioma cells following Photofrin-mediated photodynamic therapy, suggesting that down-regulating ALKBH2 expression in cancer cells may enhance the anti-cancer effectiveness of this treatment.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.