Thermo Fisher Scientific Protein A Polyclonal Antibody, Biotin
Protein A를 인식하는 Biotin 결합 Polyclonal Antibody로, Western blot, IHC, ELISA, IP 등에 사용 가능. 염소 유래 IgG로 제작되었으며, 고순도 이온 교환 크로마토그래피 정제. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| ELISA | 1:20,000–1:100,000 |
| Immunoprecipitation (IP) | 1:2,000–1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Protein A (Staphylococcus aureus) |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 10 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02M potassium phosphate (pH 7.2), 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot and freeze at -20°C or below.
- Avoid freeze/thaw cycles.
- Centrifuge if product is not clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only immediately before use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, and purified or partially purified Protein A (Staphylococcus aureus). Cross-reactivity against Protein A from other sources is unknown.
Target Information
Protein A is an Orphan-U GPCR with an unknown ligand, identified from genomic analysis of a gene-dense region at human chromosome 12p13. It appears to be expressed mainly in the brain, but its function remains unclear. Three alternatively spliced variants have been described; the full length of the third variant is yet to be determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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