Thermo Fisher Scientific Protein A Polyclonal Antibody, Biotin

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:10,000

-

Immunohistochemistry (IHC)

1:1,000-1:5,000

-

ELISA (ELISA)

1:20,000-1:100,000

-

Immunoprecipitation (IP)

1:2,000-1:10,000

-

Product Specifications

Species Reactivity

Chemical

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Protein A [Staphylococcus aureus]

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

10 mg/mL

Purification

Ion-exchange chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 10mg/mL BSA, 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Protein A [Staphylococcus aureus]. Cross reactivity against Protein A from other sources is unknown.

Target Information

Protein A is an Orphan-U GPCR with an unknown ligand. This gene was identified from genomic analysis of a gene-dense region at human chromosome 12p13. It appears to be expressed mainly in the brain. However, its function is not known. Three alternatively spliced variants have been described; the full length of the third variant is yet to be determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.